Improved tumour targeting by disulphide stabilized diabodies expressed in Pichia pastoris
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Immunogenicity of heparin-binding hemagglutinin expressed by Pichia pastoris GS115 strain
Objective(s): Heparin-binding hemagglutinin (HBHA), a mycobacterial cell surface protein, mediates adhesion to nonphagocytic cells and the dissemination of Mycobacterium tuberculosis (M. tuberculosis) from the site of primary infection. Superior expression systems are required to obtain abundant M. tuberculosis proteins for the purpose of diagnosing M. tuberculosis infection or for the immuniza...
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BACKGROUND The ascomycete Ophiostoma piceae produces a sterol esterase (OPE) with high affinity towards p-nitrophenol, glycerol and sterol esters. Its hydrolytic activity on natural mixtures of triglycerides and sterol esters has been proposed for pitch biocontrol in paper industry since these compounds produce important economic losses during paper pulp manufacture. RESULTS Recently, this en...
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Members of the six-transmembrane segment family of ion channels share a common structural design. However, there are sequence differences between the members that confer distinct biophysical properties on individual channels. Currently, we do not have 3D structures for all members of the family to help explain the molecular basis for the differences in their biophysical properties and pharmacol...
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genes. Methods Enzymol. 185:60-89. 5.Switzer, R.C., C.R. Merril and S. Shifrin. 1979. A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels. Anal. Biochem. 98:231-237. 6.Tabor, S. and C.C. Richardson. 1985. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc. Natl. Acad. Sci. USA 82:1074-1078. 7.Whela...
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Phospholipase C catalyzes the hydrolysis of the sn-3 phosphate head group in phospholipids including phosphatidylcholine, phosphatidyletanolamine, and phosphatidylserine with the formation of 1,2-diacylglycerol and water soluble phosphate esters phosphorylcholine, phosphorylethanolamine, and phosphorylserine. Phospholipase C from P. pastoris is intended for use in the refining of soybean oil an...
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ژورنال
عنوان ژورنال: Protein Engineering Design and Selection
سال: 1997
ISSN: 1741-0126,1741-0134
DOI: 10.1093/protein/10.10.1221